Central Asian Journal of Medicine


In the formation and outcome of recurrent bronchitis, the participation of the genetic characteristics of the organism, in particular, the polymorphism of the genes of some cytokines, is not excluded. One of which is tumor necrosis factor alpha. The aim of this study was to study the frequency of polymorphic alleles and genotypes G-308A of the TNF-α gene, as well as their influence on the synthesis of TNF-α in patients with RB. The study was carried out in 119 children aged 2 to 7 years with RB (main group). All patients of the main group were divided into 2 subgroups: subgroup I of 62 children with recurrent bronchitis, subgroup II consisted of 57 patients with RB on the background of LGD. The control group consisted of 110 conventio healthy children of the same age. The distribution of alleles and genotypes of the TNF-α gene in the studied groups of patients with RB and RB on the background of LGD and the control groupcorresponded to the Hardy-Weinberg equilibrium. In the active phase of RB, the level of TNFα was 6.7 times higher than in the control group. In children of RB against the background of LGD, a 4.9-fold increase in the TNFα concentration was revealed. In patients of subgroup II in the remission phase, there was a tendency towards a decrease in TNFα (P> 0.49), while in patients of subgroup I in the remission phase, the level of TNFα was significantly reduced (P <0.0001). The data obtained indicate that in RB patients against the background of LGD, the process of acute immune inflammation lasts longer. The highest frequency of the G allele and the G / G genotype was noted in the studied groups. At the same time, the indices of the G allele and the homozygous G / G genotype in patients of the II subgroup tended to decrease in comparison with the control group, i.e. this genotype has a protective effect. The minor allele A and the heterozygous G / A genotype were greatest in subgroup II (17.7% in subgroup I and 21% in subgroup II) compared to conditionally healthy children. At the same time, the chance of developing of RB carriers of the heterozygous genotype G / A in subgroup II is 1.6. Not a single case of carriage of mutant genotypes A / A was revealed among patients with RB and healthy people. The presence of the A allele was accompanied by an increase in TNFα production in RB patients against the background of LGD, regardless of the phase of the disease.

First Page


Last Page



1. Guliev N.D., Ragimova. N. D. Polymorphism of cytokine genes in newborns with intrauterine infections. \\ Russian Bulletin of Perinatology and Pediatrics, 2015 No. 6. p.42-47. 2. Zykov M.V., Makeeva O.A., Golubenko M.V. et al. Study of the rs1800629 (G308A) polymorphism of the TNFα gene in patients with ST-segment elevation myocardial infarction. Russian Journal of Cardiology 2014. No. 10. S.-13-18. 3. Mirmanova N.A. Association of genetic polymorphism of the TNFα gene (G-308) with the formation of severe and complicated forms of influenza in children. Chita State Medical Academy Chita. Volume 5, No. 4 2013 p.30-34. 4. Khotko E.A., Taganovich A.D. Polymorphism of receptor genes and their ligands in chronic obstructive pulmonary disease. Medical journal No. 3. P. 36-42. Belarus 2016. 5. Dawid S. [et al.] The signifcace of IL1β +3953C>T, IL-6 -174G>C and -596G>A, TNF-α -308G>A gene polymorphisms and 86 bp variable number tandem repeat polymorphism of IL-1RN in bronchopulmonary dysplasia in infants born before 32 weeks of gestation/ Cent Eur J Immunol. 2017: 42(3): pp.287-293 6. Hong S.J. [et al.] TNF-alpha (-308G/A) and CD14 (-159T/C) polymorphisms in the bronchial responsiveness of Korean children with asthma // J. Allergy Clin Immunol. 2007. No. 173. pp. 970-976. 7. HuGENavigator (2013). Available at: http://www.Hugenavigator.net/ HuGENavigator/ gene Prospector (accessed June 2013). 8. Hajeer A.H, Hutchinson I.V. Influence of TNF alpha gene polymorphisms on TNF alpha production and disease. Hum Immunol. 2001 Nov; 62(11):1191-9; 9. Patel J.A., Reuben Matalon Polymorphisms of immunity genes and susceptibility to otitis media in children. Published: April 9.2014. https:// doi.org/10.1371/journal.pone.0093930 10. Shevchenko A.V, Golovanova O.V, Konenkov V.I, Features of polymorphism of promoter regions of genes cytokines IL1, IL4, IL5, IL6, IL10 and TNFα at the European population of Western Siberia. Immunology 2010; 4; 176-181.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.