One of the promising methods is the use of dermal fibroblasts for the treatment of skin defects. These cells are easily cultured in the laboratory without losing their functions. Due to their key role in maintaining tissue homeostasis, fibroblasts, like no other cells, are able to effectively create conditions for the proliferation and migration of other cell types. In the world, there are a number of manufacturers that produce wound coatings based on fibroblasts in an industrial way, these are TransCyte-allogeneic fibroblasts of the human neonatal foreskin associated with a silicon membrane and grown on pig collagen covering a nylon mesh. Aim: to evaluate the prospects and safety of using a tissue-engineered construction created on the basis of boiled silk gauze and fibroblasts for the treatment of superficial skin defects. Materials and methods: An experimental study was conducted to investigate the effectiveness of a tissue-engineered construct derived from bovine collagen with flavonoids from Saphora japonica with the addition of allofibroblasts to optimize the regeneration of deep soft tissue defects on the backs of rats. Results of research: Clinical and laboratory studies of peripheral blood of white rats were performed on the BC-3000 (Mindray, P. R. China) hematological analyzer. According to a detailed blood test, the number of red blood cells and white blood cells, Hb, color index, hemogram with counting of reticulocytes, platelets, basophils, eosinophils, rod-shaped leukocytes, segmented leukocytes, lymphocytes, monocytes, the rate of blood sedimentation etc., were studied. Conclusion. The results of the research allow us to conclude that tissue-engineered construction made of boiled silk gauze and allofibroblasts is safe for the health of experimental animals in case of surface skin defects.
1. Asti A., Gioglio L. Natural and syntheticbiodegradable polymers: different scaffolds for cell expansion and tissue formation // Int J Artif Organs. –2014. – Vol. 37, N 3. – С. 187-205 2. Isabel Sánchez-Muñoz, Rosario Granados, Purificación Holguín Holgado, José Antonio García-Vela, Celia Casares, Miguel Casares. The use of adipose mesenchymal stem cells and human umbilical vascular endothelial cells on a fibrin matrix for endothelialized skin substitute. 2015 Jan;21(1-2):214-23. https://www.liebertpub.com/doi/10.1089/ten.tea.2013.0626 3. Khramova N.V., Amanullayev R.A., Makhmudov A.A. Otsenka effektivnosti tkanevoy konstruktsii pri poverkhnostnykh defektakh kozhi // Obrazovaniya // Novyy den' v meditsine №3 (35), Bukhara, 2021g., - S. 45-50. (in Russian). 4. Khramova N.V., Kholmatova M.A., Mungiyev M.Z. K voprosu ispol'zovaniya rannikh pokrytiy i kletochnykh tekhnologiy dlya optimizatsii regeneratsii kozhi // Stomatologiya (Sredneaziatskiy nauchno-prakticheskiy zhurnal). –- Tashkent, 2018. - №4 (73). - S.57-59. (in Russian). 5. Khramova N.V., Khusanova YU.B., Makhmudov A.A. Ekvivalenty kozhi: za i protiv // Zhurnal stomatologii i kraniofatsial'nykh issledovaniy. - Tashkent, 2020. - №1 (01). - S.72-75.
KHramova, Natalia V.; Xusanova, Yulduz B.; and Makhmudov, Alisher А.
"REGARDING THE REGENERATION OF SUPERFICIAL SKIN DEFECTS,"
Central Asian Journal of Medicine: Vol. 2021
, Article 11.
Available at: https://uzjournals.edu.uz/tma/vol2021/iss2/11