Background. Morphofunctional state of newborn immunity organs, in particular lymph nodes, during sepsis has not been well-studied so far. This research was dedicated to study the morphological and morphometric features of the lymph nodes of infants who died in the neonatal period because of sepsis. Methodology. The research materials were samples of paratracheal and mesenteric lymph nodes which were taken from 42 neonatal infants’ autopsy specimens. Morphological, histochemical and morphometric methods were used to study lymph node samples obtained from different localizations. Morphometric analysis was applied to evaluate the lymph node diameter, cortex (B-zone) width, paracortical T-zone as well as the medullary layer and also to determine the paracortical zone/cortex and cortex/medullary layer ratio or the coefficient. Results. The neonatal sepsis in the newborns is accompanied by intrauterine-acquired and secondary immunodeficiencies. It was revealed that in intrauterine-acquired immunodeficiency, the main morphological and functional zones of the peripheral organs of immunogenesis are shown to be immature and hypoplastic due to underpopulation of lymphocytes. Depending on the disruption level of the humoral or cell-mediated immune system, lack of lymphocyte activation in light reproduction centers as well as corresponding structural and functional zones depletion of lymph nodes occur in secondary immunodeficiencies. They are followed by zone replacement with reticular and connective tissue. Conclusions. In intrauterine sepsis, there was the lag in the main morphologic formation which is functional zones of the lymph nodes, in postnatal sepsis on the background of the secondary immunodeficiency that the devastation of the corresponding structural and functional zones of the lymph nodes was observed according to the damage of the cellular or humoral immunity.
1. Akhmedov M.D. et al., Variants and clinical evaluation of secondary immunodeficiencies in intestinal infections // Tomsk State University Journal. General practitioner. - 1999.-№ 2. P. 23-24. 2. Ivanovskaya T.E. The immune system and its relationship with the nature of the infectious process // Arch. pathology .- 1998.- No. 7.- P. 3-12. 3. Ivanovskaya T.E., Katasonova L.P. The Thymus structure, immune status and pathological process // Arch. pathology.-1986.- No. 1.- P. 3-7. 4. Kamilova A.T. et al., Correction of intestinal dysbiosis in children with small intestine enzymopathies // Honey. Journal of Uzbekistan. - 2002.- No. 2-3. -P. 44-45. 5. Mokretsova E.B. et al.. Characterization process of epithelium proliferation of colon mucous membrane in the acute period of salmonellosis. // Arch.pathology. - 2001.- T.63. P. 15-18. 6. Plaksina A. G. Pathochemical features of the critical state in children of the first year of life with purulent-inflammatory diseases Text: author thesis: 14.03.03, 03.01.04 / A. G. Plaksina. Omsk, 2010.222 p. 7. Sepsis at the beginning of the XXI century. Classification, clinical diagnostic concept and treatment. Pathologic anatomical diagnosis. Text .: practical guide / Ed. V.S. Saveliev , B.R. Gelfand. - M .: Litterra, 2006 .-- 176 p. 8. Khlystova Z.S. et al. Sequence of embedding lymphatic organs in human fetal immune system and its importance in perinatal pathology .// Arch.of patology 2002.-- number 2.- P. 16-19. 9. Shabalov N.P. Sepsis of the newborn. / N.P. Shabalov, D.O. Ivanov // Neonatology. 2006. - T. 2.P. 7 - 43. 10. Yatsyk G.V. Sepsis of the newborn. Modern problems of diagnosis and treatment Text. / G.V. Yatsyk, E.P. Bombardirova // Practice of a pediatrician. 2009 February . P. 6-9. 11. Czaja AS Readmission and late mortality after pediatric severe sepsis Text. / AS Czaja, JJ Zimmerman, L . B. Nathens // Pediatrics. 2009. - Vol. 123 (3) .- P. 849-857. 12. Muller S. et al., Intestinal intraepithelial lymphocytes exert potent protective cytotoxic activity during an acute virus infection.//J. Immmunol. 2000. Feb / 15; 164 (4): 1986-94. 13. Lawn, J.E., Blencowe, H., Oza, S., You, D., Lee, A.C., Waiswa, P., Lalli, M., Bhutta, Z., Barros, A.J., Christian, P. and Mathers, C., 2014. Every Newborn: progress, priorities, and potential beyond survival. The Lancet, 384(9938), pp.189-205. 14. Stoll, B.J., Hansen, N.I., Bell, E.F., Walsh, M.C., Carlo, W.A., Shankaran, S., Laptook, A.R., Sánchez, P.J., Van Meurs, K.P., Wyckoff, M. and Das, A., 2015. Trends in care practices, morbidity, and mortality of extremely preterm neonates, 1993-2012. Jama, 314(10), pp.1039-1051. 15. Bernard Kan, Hamid Reza Razzaghian, Pascal M. Lavoie, An Immunological Perspective on Neonatal Sepsis, Trends in Molecular Medicine, Volume 22, Issue 4, 2016, Pages 290-302. 16. Brubaker, S.W., Bonham, K.S., Zanoni, I. and Kagan, J.C., 2015. Innate immune pattern recognition: a cell biological perspective. Annual review of immunology, 33, pp.257-290. 17. Sadeghi, K., Berger, A., Langgartner, M., Prusa, A.R., Hayde, M., Herkner, K., Pollak, A., Spittler, A. and Förster-Waldl, E., 2007. Immaturity of infection control in preterm and term newborns is associated with impaired toll-like receptor signaling. The Journal of infectious diseases, 195(2), pp.296-302. 18. PrabhuDas, M., Bonney, E., Caron, K., Dey, S., Erlebacher, A., Fazleabas, A., Fisher, S., Golos, T., Matzuk, M., McCune, J.M. and Mor, G., 2015. Immune mechanisms at the maternal-fetal interface: perspectives and challenges. Nature immunology, 16(4), pp.328-334.
Allaberganov, Dilshod Sh.; Israilov, Radjab I.; and Tajimova, Nargiza I.
"PATHOMORPHOLOGY OF LYMPHATIC NODES DURING NEONATAL SEPSIS,"
Central Asian Journal of Medicine: Vol. 2020
, Article 2.
Available at: https://uzjournals.edu.uz/tma/vol2020/iss2/2