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Central Asian Journal of Medicine

Abstract

Objective: to study the relationship of the C3435T polymorphism of the MDR1 gene with the presence of resistance to methotrexate treatment in patients with rheumatoid arthritis. Materials and Methods: In our study, 76 patients with rheumatoid arthritis and 24 practically healthy volunteers took part. Polymorphism of the C3435T gene was determined using the polymerase chain reaction (PCR), which was carried out in a 7500 Fast Real Time PSR Systems (USA) amplifier. Results: According to the results of our studies, methotrexate resistance was detected in patients with SS genotype C3435T polymorphism of the MDR1 gene. In contrast, carriers of TT genotype showed high sensitivity to treatment and low disease activity. Conclusions: The interrelation of the C3435T polymorphism of the MDR1 gene with the presence of resistance to methotrexate treatment in patients with RA was established. Conducting genotyping of patients with rheumatoid arthritis before the appointment of methotrexate makes it possible to predict the presence of resistance to this drug.

First Page

62

Last Page

66

References

1. Bulatović M. et al. Prediction of clinical non-response to methotrexate treatment in juvenile idiopathic arthritis // Ann. Rheum. Dis. – 2012. – Т. 71, №9. – P. 1484-1489. 2. Chen J., Chen L., Mao N., Liu Y. Association of the MDR1 3435 polymorphism in patients with refractory rheumatoid arthritis in a Chinese population // Rheumatol. Int. – 2012. – №12. – P. 127-3130. 3. Drozdzik M., Rudas T. et al. The effect of 3435C>T MDR1 gene polymorphism on rheumatoid arthritis treatment with disease-modifying antirheumatic drugs // Europ. J. Clin. Pharmacol. – 2006. – Vol 62. – P. 933-937. 4. Grabar P.B., Logar D. et al. Genetics determinants of methotrexate toxicity in rheumatoid artritis patients: a study of polymorphisms affecting methotrexate transport and folate metabolism // Eurор. J. Clin. Pharmacol. – 2008. – Vol. 64. – P. 1057-1068. 5. Guo Q., Wang Y. et al. Rheumatoid arthritis: pathological mechanisms and modern pharmacologic therapies // Bone Res. – 2018. – Vol. 6. – P. 2-15. 6. Muralidharan N., Antony P. et al. Multidrug resistance 1 (MDR1) 3435C>T gene polymorphism influences the clinical phenotype and methotrexate-induced adverse events in South Indian Tamil rheumatoid arthritis // Europ. J. Clin. Pharmacol. – 2015. – Vol. 71. – P. 959-965. 7. Pawlik A., Wrzesniewska J., Fiedorowicz-Fabrycy I., Gawronska-Szklarz B. The MDR1 3435 polymorphism in patients with rheumatoid arthritis // Int. J. Clin. Pharmacol. Ther. – 2004. – Vol. 42, №9. – P. 496-503. 8. Sharma S. et al. Interaction of genes from influx-metabolism-efflux pathway and their influence on methotrexate efficacy in rheumatoid arthritis patients among Indians // Pharmacogen. Genom. – 2008. – Vol. 18, №12. – P. 1041-1049. 9. Samara S.A., Irshaid Y.M. et al. Association of MDR1 C3435T and RFC1 G80A polymorphisms with methotrexate toxicity and response in Jordanian rheumatoid arthritis patients // Int. J. Clin. Pharmacol. Ther. – 2014. – Vol. 52, №9. – P. 746-755. 10. Takatori R. et al. ABCB1 C3435T polymorphism influences methotrexate sensitivity in rheumatoid arthritis patients // Clin. Exp. Rheumatol. – 2006. – Vol. 24, №5. – P. 546-554.

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