A highly efficient HPLC method has been developed for the simultaneous quantitative analysis of seven ginsenosides, Rb1, Rb2, Rc, Rd, Re, Rf and Rg1. Chromatographic separation of analytes was achieved in less than 20 minutes using a polyvinyl alcohol bonded column, with UV detection at 203 nm in the isocratic mode of the mobile phase, consisting of acetonitrile and water-82.5/17.5 v/v. The developed method was tested in the concentration range of 10-120 μg/ml analytes. Accuracy based on the values of the nominal concentration was in the range of 98.7–100.8%. The detection limit was from 0.43 to 1.03 μg/ml, and the quantification limit was from 1.42 to 3.13 μg/ml.
1. Yamaguchi H., Matsuura H., Kasai R., Tanaka O., Satake M., Kohda H., Izumi H., Nuno M., Katsuki S., Isoda S., Shoji J.,Goto K.Analysis of Saponins of Wild Panax ginseng. Chem Pharm Bull.–1988, Vol 36,-P.4177–4188. 2. Liu C.X.,Xiao P.G. Recent advances on ginseng research in China.J.Ethnopharmacol.– 1992, Vol. 36,-P. 27–38. 3. Park M.K., Park J.H., Han S.B.,Shin Y.G.,Park I.H. High-performance liquid chromatographic analysis of ginseng saponins using evaporative light scattering detection. J.Chromatography A.–1996, Vol. 736,-P. 77–81. 4. Validation of analytical procedures: methodology ICH harmonised tripartite guideline, having reached step 4 of the ICH process at the ICH Steering Committee meeting on 6 November.–1996. 5. Novotna K.,Havlis J.,Havel J.Optimisation of high performance liquid chromatography separation of neuroprotective peptides:Fractional experimental designs combined with artificial neural networks.J.Chromatography A.–2005, Vol. 1096,- P. 50–57. 6. Kaiser R. Gas Chromatographie, Geest und Portig, Leipzig.–1959,-P.33–34. 7.Lou D.W.,Saito Y.,Jinno K. Simultaneous LC Determination of Ginsenosides Using a Modified Extraction Procedure and an Improved Step Gradient Program, Chromatographia.–2005, Vol. 63(1-2),-P. 31–35.
Soliev, Azamjon Bakhodirovich
"DEVELOPMENT OF HPLC METHOD FOR THE SEPARATION OF
GINSENOZIDES USING POLYVINYL ALCOHOL BONDED STATIONARY
Scientific Bulletin of Namangan State University: Vol. 1
, Article 18.
Available at: https://uzjournals.edu.uz/namdu/vol1/iss7/18