Scientific Bulletin of Namangan State University


A highly efficient HPLC method has been developed for the simultaneous quantitative analysis of seven ginsenosides, Rb1, Rb2, Rc, Rd, Re, Rf and Rg1. Chromatographic separation of analytes was achieved in less than 20 minutes using a polyvinyl alcohol bonded column, with UV detection at 203 nm in the isocratic mode of the mobile phase, consisting of acetonitrile and water-82.5/17.5 v/v. The developed method was tested in the concentration range of 10-120 μg/ml analytes. Accuracy based on the values of the nominal concentration was in the range of 98.7–100.8%. The detection limit was from 0.43 to 1.03 μg/ml, and the quantification limit was from 1.42 to 3.13 μg/ml.

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