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Scientific Bulletin of Namangan State University

Abstract

Size-exclusion chromatography (SEC) equipped with refractive index (RI) and multiangle laser light scattering (MALLS) detectors were applied to evaluation of charged molecules content in a series of polysaccharide derivatives with different average molar mass (MM) values. It was shown that a minor amount of residual charged groups and neutral fractions of larch arabinogalactans (AG) were separated from neutral molecules in various concentrations of the injected samples. Electrostatic effects in separation of carboxymethylcelluloses (CMC) were demonstrated by concentration dependence of retention volume at various injected sample concentrations.

First Page

96

Last Page

100

References

1. A.Boymirzaev, Chemistry of raw material. 2 (2009) 19-28 2. S.C.Churms. Journl of Chromatography Library.66 (2002) 267-303. 3. T.Song, A.Pranovich, B.Holmbom. Bioresource Technology (2013) 198-203. 4. X. Michael Liu, Wei Gao, E. Peter Maziarz, Joseph C. Salamone, John Duex and Erning Xia. J. Chromatog. A ,2006, v.1104, 1-2, pp. 145-153 5. C.W.Hoogendam, A.de Keizer, M.A.Cohen Stuart, B.H.Bijsterbosch, J.A.M.Smit, J.A.P.P.van Dijk, P.M. van der Horst, J.G. Batelaan. Macromolecules, 1998, v.31, p.6297.

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