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CHEMISTRY AND CHEMICAL ENGINEERING

CHEMISTRY AND CHEMICAL ENGINEERING

DOI

10.34920/cce2022112

Abstract

The aim of the study was to optimize the process of extraction of aflatoxins secreted by fungi of the genus Aspergillus from almonds, as well as to analyze the content of aflatoxins using high performance liquid chromatography (HPLC). An HPLC LC 2030 C3D Plus (Shimadzu) column Shim-pack GIST-HP C18 150x4.6 mm 3 µm (Shimadzu) was used at a ratio of H2O/H3C-CN = 40/60 as an eluent at a thermostat temperature of 40 degrees Celsius. Based on the analysis on a fluorescent detector RF-20A with an excitation wavelength of 365-450 nm and a flow rate of 0.5 ml/min, a directed sample volume of 10 μl, the amount of natural compounds AFB1, AGB2, AFG1, AFG2 of the aflatoxin group was determined. It has been established that the content of aflatoxins does not exceed the permissible limits and differs depending on the area of cultivation. Almonds grown in the Syrdarya region contain one and a half times more aflatoxin G2 than almonds grown in the Jizzakh region, and four times more than almonds grown in the Samarkand region.

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