The aim of this study is determination of optimal fermentation conditions of the Pichia pastoris yeast expressing the recombinant Pres2-S protein of hepatitis B virus (HBV). For this purpose we investigated the main factors influencing for the cultivation of recombinant strain of the yeast Pichia pastoris pPIC3,5-S-HBsAg - the composition of the nutrient medium, pH and dissolved oxygen (DO). As a result, by enzyme-linked immunosorbent assay (ELISA) the optimal concentration of methanol ≈1.0 %, which is the initiator and the only source of carbon for protein expression in the Mut+ phenotype Pichia pastoris yeast cells was determined. In the selected conditions at the end of the fermentation process, the wet biomass of yeast cells was 420 g/l.
Sobirdjan, SASMAKОV; Оybek, ASHIRОV; Jaloliddin, ABDURAKHMANОV; and Shuhrat, XASANОV
"OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN
(M-HBsAg) OF HEPATITIS B VIRUS (HBV),"
CHEMISTRY AND CHEMICAL ENGINEERING: Vol. 2020
, Article 10.
DOI: 10.51348/M PAU8653
Available at: https://uzjournals.edu.uz/cce/vol2020/iss3/10