The methods of oriented immobilization of lipases on a solid carrier - Silochrome C-80 have been developed. The resulting sorbents were tested in the process of obtaining biodiesel from cotton oil. It was shown that immobilized lipase on Silochrome C-80 with NH2 group has the highest transetrification ability and stability, while in sorbents with the enzyme immobilized with -COOH and -SH groups this value was 87 and 53%, respectively. Biocatalysis using an affinity sorbent of immobilized lipase with the –NH2 group has a high operational stability, which, after 240 hours of operation, retains more than 50%, and after 480 hours, 17% retains its initial activity.
Jamolitdin Fazlitdinovich, Ziyavitdinov; Utkir Kudratillayevich, Inogamov; Nodir Sharifovich, Berdiev; and Uchkun Jomuradovich, Ishimov
"Development of a method of oriented immobilization of lipases on a solid carrier,"
Chemistry and Chemical Engineering: Vol. 2019
, Article 31.
Available at: https://uzjournals.edu.uz/cce/vol2019/iss3/31