Acta of Turin Polytechnic University in Tashkent


Experiments have been carried out to study the effect of violasein on the activity of various protein kinases. It was shown that violacein inhibits the protein kinase C (PKC) subfamily of the classical type, as well as some novel and atypical forms of PKC enzymes. Data on the inhibition of the activity of the catalytic subunits of protein kinase A and protein kinase C by violasein activity testify to the direct interaction of violacein with these sites of the complex enzyme molecule. Kinetic analysis of inhibition of PKA by violacein showed that inhibition occurs in a mixed type with respect to ATP. There was also a slight inhibition of Ca2+/calmodulin-dependent protein kinase and the absence of the effect of violasein on the activity of protein tyrosine kinase (Src). A possible analogy in the mechanism of action of violacein with a known serine-threonine kinase inhibitor, staurosporin, is discussed.

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